产品货号：
GS2118
中文名称：
AspS9I内切酶
英文名称：
AspS9I
产品规格：
500U
发货周期：
1～3天
产品价格：
询价
储存条件：冷冻（-20℃）
技术规格
相关搜索：AspS9I内切酶，AspS9I限制性内切酶
技术规格
| Unit definition | One unit of the enzyme is the amount required to hydrolyze 1μgof Lambda DNA in 1hourat 37℃ in a total reaction volume of 50μl. |
| Recognition sites | G↑GNCC |
| 来源 | Arthrobacter species S9 |
| Assayed on | Lambda DNA |
| Optimal buffer | W(10mMTris-HCl(pH 8.5 at 25℃);10mMMgCl2;100 mM NaCl;1 mM DTT.) |
| Optimal temperature | 37℃ |
| Storage conditions | 10mMTris-HCl(pH 7.5);50 mM KCl;0.1 mM EDTA;7 mM 2-mercaptoethanol;200 μg/ml BSA;50%glycerol;Store at -20℃. |
| Ligations | After 20-fold overdigestion with enzyme more than 90%of the DNA fragments can be ligated and recut. |
| Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1μgof Lambda DNA with 30 u.a.of enzyme for 16hoursat 37℃. |
| Methlation effect | Bloked by overlapping Dcm methylation(CmCWGG):GGNCCWGG |
| Thermal inactivation | enzyme is inactivated by incubation at 65℃ for 20 mins. |
| Campatible Ends | AsuI,BmgT120I^,BsiZI,BspLI^,Cfr13I,FmuI^,PspPI,Sau96I,UnbI^ |
相关搜索：AspS9I内切酶，AspS9I限制性内切酶